Journal: International Journal of Molecular Sciences
Article Title: Serum and Tissue Light-Chains as Disease Biomarkers in AL Amyloidosis
doi: 10.3390/ijms26199511
Figure Lengend Snippet: Normal range of kappa/lambda FLC ratio Freelite ® assay. The serum quantification of free light chains (FLCs) and the kappa/lambda ratio vary according to renal function. ( a ) A normal kappa/lambda ratio in case of normal renal function (0.26–1.65) has a 100% negative predictive value for light-chain cardiac amyloidosis (AL-CA). If a kidney disease is present, the normal range is 0.37–3.10. ( b ) Comparison of two widely used serum free light chain (sFLC) assays. Freelite ® (The Binding Site, Birmingham, UK) uses polyclonal antibodies and is available on multiple clinical chemistry analyzers. N Latex FLC (Siemens Healthineers, Erlangen, Germany) employs a monoclonal antibody mixture and is available exclusively on Siemens nephelometric platforms.
Article Snippet: Sebia FLC ELISA kappa/lambda , Polyclonal , ELISA sandwich , AP22 Elite , - Larger measurement interval - Reduced repetitions needed for FLC quantification , - Proportional positive bias at high concentrations , - Quantitative with improved linearity across broad ranges.
Techniques: Comparison, Binding Assay